Cloning vector
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A Versatile Zero Background T-Vector System for Gene Cloning and ...Because it is difficult to generate a high-quality TA cloning vector in individual ... Chen S, Tao L, Zeng L, Vega-Sanchez ME, Umemura K, Wang GL (2006b) A ...A novel series of high-efficiency vectors for TA cloning and blunt-end ...2019年4月23日 · I constructed three plasmid vectors for PCR cloning in this study (Fig. 1). In pCRT (2,728 bp) for TA cloning, T-overhang vector for cloning of dA- ...Improved electroporation and cloning vector system for gram ...This vector features a multiple cloning site region flanked by E. coli transcription termination ... The vector and electroporation system was also used to clone directly into E. faecalis. ... [PubMed] [Google Scholar]; Macrina FL, Evans RP, Tobian JA, Hartley DL, Clewell DB, Jones KR. ... Twitter · Share on Google Plus Google+.Vaccinia virus: a selectable eukaryotic cloning and expression vectordoi: 10.1073/pnas.79.23.7415. Authors. M Mackett, G L Smith, B Moss. tw | twGateway Entry Clones | Thermo Fisher Scientific - TWSeveral pENTR vectors are available for Directional TOPO cloning and direct access to the multitude of Gateway expression vectors. pCR8/GW/TOPO Vector kits. | TA Cloning Kits | Thermo Fisher Scientific - TWThe linearized vectors supplied in TA cloning kits have a complimentary 3´ deoxythymidine (T) residues allowing the insert to ligate into the vector efficiently. | Standardized cloning vectors for protein production and generation ...2018年3月16日 · Here, we modified the multiple cloning sites from commonly used expression vectors to create a new suite of cloning plasmids that simplify and ...Human PDE4B cDNA ORF Clone in Cloning Vector, HG11527-M ...Card G.L.et al., 2005, Nat. Biotechnol. 23:201-7. Wang H.et al., 2007, Biochem. J. 408:193-201. Hamblin ...圖片全部顯示Cumulated Index MedicusLee TL see Tweed WA vivo adenoviral vector - mediated gene transfer into ... Pyriform sinus perforation secondary to Purification and molecular cloning of ... Masai M , Shimazaki Lee TH see Kearney DJ Lee TW see Jensen GL J. Effect of ...
延伸文章資訊
- 1什麼是載體?
載體,在流行病中是指疾病的攜帶者和傳播者,又稱為病媒;而在分子生物學和遺傳 ... 外源DNA運送至宿主的DNA;二是利用它在宿主細胞內對目標基因進行大量複製。 ... 的訊息RNA,使用反逆轉...
- 2Gene Cloning | 如何將目標基因克隆到載體中(下) - ACE Biolabs
Gene Cloning | 如何將目標基因克隆到載體中(上) 三、目標片段連接到「載體」上:分成純化目標片段和連接反應。 純化目標片段DNA: 經由 ...
- 3重組DNA-基因轉殖的關鍵技術A
選殖. 利用載體的特定基因進行選殖。 例如:若載體帶有特定抗抗生素基因,則可在培養基中加入抗生素,若細菌能在. 含有抗生素的 ...
- 4運載體- 維基百科,自由的百科全書 - Wikipedia
又稱載體。在分子選殖中,運載體是用作人工攜帶外來遺傳物質進入另一細胞的載體的DNA分子,其中它可以被複製和/或被表現(例如質粒,黏質體,λ ...
- 5細菌、酵母菌與植物間的基因選殖技術(上) | 科學Online
依照載體目的主要可分為cloning vector及expression vector。因為vector都會隨著宿主DNA複製及細胞分裂,故其中cloning vector主要是用來選殖 ...